1131 Contraction of collagen matrices by meshwork cells

Purpose: To determine whether mobile trabecular meshwork cell$ can contract collagen matrices in vitro Methods: Bovine and human meshwork cells were cultured and seeded into or onto type I collagen matrices in sterile conditions. The tloating matrices were measured on a daily basis to determine redtlction in diameter over time. Results: The meshwork cells contracted the collagen gells in a time and cell density dependent manner. Gel contraction was produced as a direct result of meshwork cell migration. However meshwork cells were less effective at gel contraction than iibroblasts. With 250,000 cells the ID,, for meshwork cells was around 5 days and for fibroblasts lust over 2 days Conclusions: Migratory meshwork cells generate traction forces and this is a possible mechanism by which mobile proliferating meshwork cells open up the outflow pathways following Argon Laser trabeculoplasty.